ThousandsSeparator Property
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Application.ThousandsSeparator Property (Excel)

Office 2013 and later

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Sets or returns the character used for the thousands separator as a String. Read/write.

expression .ThousandsSeparator

expression A variable that represents an Application object.

This example places "1,234,567.89" in cell A1 then changes the system separators to dashes for the decimals and thousands separators.

Sub ChangeSystemSeparators() 
 Range("A1").Formula = "1,234,567.89" 
 MsgBox "The system separators will now change." 
 ' Define separators and apply. 
 Application.DecimalSeparator = "-" 
 Application.ThousandsSeparator = "-" 
 Application.UseSystemSeparators = False 
End Sub
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